Session Index

Biophotonics and Biomedical Imaging

Biophotonics and Biomedical Imaging II
Thursday, Dec. 3, 2020  16:20-17:50
Presider: Prof. Meng-Tsan Tsai
Prof. Kung-Bin Sung
Room: 6AB Room 526
16:20 - 16:35 Manuscript ID.  0063
Paper No.  2020-THU-S0602-O001
Chuan-Bor Chueh
Award Candidate
Multi-Scale Wide Field-of-View Optical Coherence Microscopy with 1.7-μm Swept Source Laser
Chuan-Bor Chueh;Ching-Yu Wang;Huai-Jen Liu;Ting-Yen Tsai;Teng-Chieh Chang;Ting-Hao Chen;Yin-Peng Huang;Yi-Ping Hung;Hsiang-Chieh Lee

We have developed a multi-scale long-wavelength optical coherence microscopy imaging system allowing three-dimensional imaging of biological tissue. Besides, we demonstrated the ability of our self-developed automatic mosaic stitching algorithm.

16:35 - 16:50 Manuscript ID.  0435
Paper No.  2020-THU-S0602-O002
Chong_Wei Kao
Award Candidate
Automatic Retinal Layer Segmentation in OCT Images Based on Deep Learning
Chong_Wei Kao;Chieh-En Lee;Jin-Shing Tseng;Chu-Ming Cheng;Chung-Hao Tien

Precise retinal layer segmentation in optical coherence tomography (OCT) helps early clinical diagnosis of some diseases. In this work, we established a segmentation algorithm for the retinal layer boundaries based on deep learning. Our method successfully segments three retinal layers with acceptable errors compared to traditional methods.

16:50 - 17:05 Manuscript ID.  0374
Paper No.  2020-THU-S0602-O003
Ying-Ting Huang
Label-free Histopathology of Alzheimer’s Disease Human Brain by Harmonic Generation Microscopy
Ying-Ting Huang;Sandeep Chakraborty;Sung-Tsang Hsieh;Ming-Jang Chiu;Hwan-Ching Tai;Chi-Kuang Sun

Here, we demonstrated a label-free, sub-micron multi-harmonic generation microscopy to elucidate the histopathological hallmarks of Alzheimer’s disease human brain ex vivo. Our harmonic generation microscopy has shown the capability to differentiate simultaneously structures of brain viz. axons, soma along with AD pathological hallmarks: Aβ-plaques and neurofibrillary tangles.

17:05 - 17:20 Manuscript ID.  0429
Paper No.  2020-THU-S0602-O004
Hsiao-Chieh Tseng
Human Intra-epidermal Nerve Fiber Imaging using Stain-free Trauma-free in vivo Harmonic Generation Microscopy
Hsiao-Chieh Tseng;Pei-Jhe Wu;Chi-Kuang Sun;Yi-Hua Liao

Diabetic neuropathy is diagnosed by skin biopsy to quantify intra-epidermal nerve fiber. Our recent study indicated the capability of third-harmonic-generation microscopy (THGM) to image intra-epidermal free-nerve endings. In this study, we successfully apply the stain-free trauma-free THGM to in vivo evaluate epidermal innervation by quantifying the number of nerve fibers.

17:20 - 17:35 Manuscript ID.  0709
Paper No.  2020-THU-S0602-O005
Chi-Hao Luo
Optically sectioned mechanical-scan-free endomicroscopy with hybrid illumination
Chi-Hao Luo;Haw Hsiao;Yuan Luo;Kuang-yuh Huang

Optical sectioning in our system is achieved by HiLo imaging technique. High speed hybrid illumination for background elimination is generated by a digital micro-mirror device (DMD),with electrically tunable lens (ETL) as a mechanical-scan-free device. Our system offers multiple advantages, which may enable large scale dynamic imaging of biological tissues.

17:35 - 17:50 Manuscript ID.  0381
Paper No.  2020-THU-S0602-O006
Pei-Jhe Wu
Stain-free Imaging of Human Intra‐epidermal Free Nerve Endings by using Third Harmonic Generation Microscopy
Pei-Jhe Wu;Hsiao-Chieh Tseng;Sung-Tsang Hsieh;Yi-Hua Liao;Chi-Kuang Sun

We demonstrate the first observation of the intra‐epidermal free-nerve-endings (IFNEs) with histopathology details by third-harmonic-generation on ex vivo human skin. With the high image contrast, noninvasiveness, high resolution, high penetration of harmonic generation microscopy, we aim to label-free slide-free in vivo image IFNEs for the peripheral neuropathy diagnosis.